Occurrence and distribution of glycerol dialkyl glycerol tetraethers in a French peat bog

The present study was aimed at examining the distribution and abundance of glycerol dialkyl glycerol tetraethers (GDGTs) of archaeal and bacterial origin in peat samples from surface and deep (ca. 50 cm) horizons of a peat bog in the Jura Mountains (northeastern France). Two principal types of GDGTs are present: extractable GDGTs, recoverable by solvent extraction, and non-extractable GDGTs, linked to the soil matrix. Within the extractable pool, “free” (i.e. core lipids) and “bound” (i.e. intact polar and/or ester-bound lipids) GDGTs can be distinguished. Extractable “free” and “bound” GDGTs were extracted using both accelerated solvent extraction (ASE) and a modified Bligh and Dyer technique. Both methods were shown to allow adequate extraction of “free” archaeal and bacterial GDGTs from soil samples. Both extraction protocols afforded similar relative distributions of archaeal and bacterial GDGTs, although poorer extraction of “bound” GDGTs was observed for ASE relative to Bligh and Dyer. Even though only low amounts of bacterial GDGTs were released after acid hydrolysis of solvent-extracted samples, non-extractable and total extractable GDGTs showed different distribution patterns in some samples. Consequently, ∗ Corresponding author. Tel : + 33-144-273-514 fax : +33-144-275-150. E-mail address: [email protected] (S. Derenne). in su -0 04 60 43 3, v er si on 1 12 M ar 2 01 0 Author manuscript, published in "Organic Geochemistry 41, 6 (2010) 559-572" DOI : 10.1016/j.orggeochem.2010.02.015